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Bio-Techne corporation
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ATCC
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Image Search Results
Journal:
Article Title: Lentiviral delivery of HIV-1 Vpr protein induces apoptosis in transformed cells
doi:
Figure Lengend Snippet: Vpr preferentially kills rapidly dividing cells. (a) Human fibroblasts were grown in Iscove’s plus 1%-depleted FCS or 10% FCS. Cells (2 × 104) were plated on day 0 and counted on days 1, 3, 5, 7, and 9. (b) Normal human fibroblasts were grown in 10% FCS and mock-infected (⧫) or infected with HIV-1NL4–3Thyenv(-) (■), HR’Thy(Vpr−) (▴), or HR’Thy(Vpr+) (×). At 144 h postinfection, infection with HIV-1NL4–3Thyenv(-), HR’Thy(Vpr−), and HR’Thy(Vpr+) resulted in 63%, 66%, and 71% Thy 1.2-positive cells, respectively. At 72, 144, and 216 h postinfection, Thy 1.2-positive cells were analyzed for annexin V staining. (c) Normal human fibroblasts were grown in 1% depleted FCS and mock-infected (⧫) or infected with HIV-1NL4–3Thyenv(-) (■), HR’Thy (Vpr−) (▴), or HR’Thy(Vpr+) (×). At 144 h postinfection, infection with HIV-1NL4–3Thyenv(-), HR’Thy(Vpr−), and HR’Thy(Vpr+) resulted in 48%, 65%, and 82% Thy 1.2-positive cells, respectively. At 72, 144, and 216 h postinfection, Thy 1.2-positive cells were analyzed for annexin V staining.
Article Snippet:
Techniques: Infection, Staining